Journal: Scientific Reports

Article Title: Speckleplethysmographic (SPG) Estimation of Heart Rate Variability During an Orthostatic Challenge

doi: 10.1038/s41598-019-50526-0

Figure Lengend Snippet: An in vivo comparison of ECG (black), SPG (blue), and PPG (red) waveforms measured from a single subject. Because of the pulse transit time from the chest (ECG) to the finger (SPG/PPG), the ECG has its R peak before the SPG peak. SPG peaks before PPG.

Article Snippet: For wireless ECG acquisition at 2000 Hz, we used a Nomadix Wireless Receiver with ECG Amplifier (BIOPAC Systems, Inc., Goleta, CA).

Techniques: In Vivo, Comparison

Journal: Scientific Reports

Article Title: Speckleplethysmographic (SPG) Estimation of Heart Rate Variability During an Orthostatic Challenge

doi: 10.1038/s41598-019-50526-0

Figure Lengend Snippet: Corrected Artifacts (n = 17).

Article Snippet: For wireless ECG acquisition at 2000 Hz, we used a Nomadix Wireless Receiver with ECG Amplifier (BIOPAC Systems, Inc., Goleta, CA).

Techniques:

Journal: Scientific Reports

Article Title: Speckleplethysmographic (SPG) Estimation of Heart Rate Variability During an Orthostatic Challenge

doi: 10.1038/s41598-019-50526-0

Figure Lengend Snippet: A box plot comparison of the percentage of artifacts corrected for ECG (green), SPG (blue), and PPG (black) during the orthostatic challenge (n = 17). The circles represent outliers. Motion artifact and noise negatively impacted the peak detection algorithm accuracy, especially for PPG.

Article Snippet: For wireless ECG acquisition at 2000 Hz, we used a Nomadix Wireless Receiver with ECG Amplifier (BIOPAC Systems, Inc., Goleta, CA).

Techniques: Comparison

Journal: Scientific Reports

Article Title: Speckleplethysmographic (SPG) Estimation of Heart Rate Variability During an Orthostatic Challenge

doi: 10.1038/s41598-019-50526-0

Figure Lengend Snippet: A comparison of SPG, PPG, and ECG HRV time domain parameters (SDNN; ms and RMSSD; ms) for the 17 subjects during both sitting and standing conditions. SPG and PPG are compared to ECG on scatter plots with a line of best fit and the line of best fit equation, the Pearson’s correlation coefficient, and significance in the upper left corner of the plot. Each Bland-Altman plot corresponds to the scatter plot directly above it, with the mean difference (black line), the 95% upper and lower limits of agreement (blue dashed lines), and the acceptable upper and lower limits of agreement (red dashed lines) also plotted. It should be noted that the Bland-Altman plots for SPG and PPG have different y-axis scales.

Article Snippet: For wireless ECG acquisition at 2000 Hz, we used a Nomadix Wireless Receiver with ECG Amplifier (BIOPAC Systems, Inc., Goleta, CA).

Techniques: Comparison

Journal: Scientific Reports

Article Title: Speckleplethysmographic (SPG) Estimation of Heart Rate Variability During an Orthostatic Challenge

doi: 10.1038/s41598-019-50526-0

Figure Lengend Snippet: A comparison of SPG, PPG, and ECG HRV frequency domain parameters (LF; ms 2 and HF; ms 2 ) for the 17 subjects during both sitting and standing conditions. SPG and PPG are compared to ECG on scatter plots with a line of best fit and the line of best fit equation, the Pearson’s correlation coefficient, and significance in the upper left corner of the plot. Each Bland-Altman plot corresponds to the scatter plot directly above it, with the mean difference (black line), the 95% upper and lower limits of agreement (blue dashed lines), and the acceptable upper and lower limits of agreement (red dashed lines) also plotted. It should be noted that some of the Bland-Altman plots for SPG and PPG have different y-axis scales.

Article Snippet: For wireless ECG acquisition at 2000 Hz, we used a Nomadix Wireless Receiver with ECG Amplifier (BIOPAC Systems, Inc., Goleta, CA).

Techniques: Comparison

Journal: Scientific Reports

Article Title: Speckleplethysmographic (SPG) Estimation of Heart Rate Variability During an Orthostatic Challenge

doi: 10.1038/s41598-019-50526-0

Figure Lengend Snippet: A comparison of additional SPG, PPG, and ECG HRV frequency domain parameters (normalized HF; normalized units (n.u.) and LF/HF; ratio) for the 17 subjects during both sitting and standing conditions. SPG and PPG are compared to ECG on scatter plots with a line of best fit and the line of best fit equation, the Pearson’s correlation coefficient, and significance in the upper left corner of the plot. Each Bland-Altman plot corresponds to the scatter plot directly above it, with the mean difference (black line), the 95% upper and lower limits of agreement (blue dashed lines), and the acceptable upper and lower limits of agreement (red dashed lines) also plotted.

Article Snippet: For wireless ECG acquisition at 2000 Hz, we used a Nomadix Wireless Receiver with ECG Amplifier (BIOPAC Systems, Inc., Goleta, CA).

Techniques: Comparison

Journal: Scientific Reports

Article Title: Speckleplethysmographic (SPG) Estimation of Heart Rate Variability During an Orthostatic Challenge

doi: 10.1038/s41598-019-50526-0

Figure Lengend Snippet: Subjects remained seated for 5 minutes while a finger-clip device simultaneously collected SPG and PPG data and a wireless system collected ECG data. After a 30 second transition period for the subject to go from sitting to standing, 5 minutes of SPG, PPG, and ECG data were collected while the subject remained standing.

Article Snippet: For wireless ECG acquisition at 2000 Hz, we used a Nomadix Wireless Receiver with ECG Amplifier (BIOPAC Systems, Inc., Goleta, CA).

Techniques:

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Adult zebrafish ECG recording apparatus and protocol. (A) Comparison of human and zebrafish ECG. Top: ECG of a healthy 32-year-old human male, obtained from the MIT-BIH Normal Sinus Rhythm Database . Bottom: ECG from a 6-month-old adult male AB zebrafish, captured using the described recording apparatus and protocol. All cycles within the 60 s (human) and 120 s (zebrafish) recordings were overlaid (green traces), and an average trace was calculated (black trace) using the LabChart ‘ECG Analysis’ module. Traces are shown on the same time scale for direct comparison. (B) Adult zebrafish ECG apparatus; during recordings, the zebrafish is placed ventral side up within the mold located in a well on the base. For ease of reference, apparatus components and axes used to describe the movement of the components are labeled. (C) Summary of adult zebrafish ECG recording protocol. (D) Example from a 6-month-old adult male AB zebrafish using the described recording apparatus and protocol. The trace image was captured using LabChart ‘Zoom View’.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Comparison, Labeling

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Overview of zebrafish ECG reading GUI (zERG), a zebrafish ECG analysis software program. In step 1, ECG voltage measurements are imported into zERG. In step 2, data for peak analysis are selected. During peak analysis, P waves (red asterisks) and QRS complexes (black asterisks) are identified based on the following: (1) the user-defined wave amplitude threshold; (2) the minimum peak distance; and (3) classification based on the distance between the P peak to R wave of consecutive cycles and the reference distance defined as two times the first ‘P peak to R wave’ interval. Consecutive red or black peaks are marked yellow for error correction; the user can then manually add and/or delete P waves and/or QRS complexes. Once all waves are marked correctly, the user can proceed to step 3 by choosing the ‘Calculate Average Trace’ button. This plots an average trace and allows the user to click on ‘Add Markers’ to note wave markers on the average trace. Finally, after all peaks are confirmed, the user selects ‘Analyze ECGs’ to obtain a graphical user interface (GUI) table with the ECG metrics, in addition to an output .txt and several average trace plots. The yellow boxes show an example of a trace in which the P wave amplitude exceeds the R wave amplitude and how zERG handles such traces. The example trace in the blue boxes was captured from a 6-month-old AB wild-type male fish, and the trace in the yellow boxes was captured from a 3.5-month-old AB wild-type male fish after being dosed with 0.800 mmol/l FA for 15 min. All images were captured from zERG version 1.0.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Software

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Comparison of ECG metrics between zERG and LabChart. Measurements from a total of 55 traces from two independent experiments, either from 0.800 mmol/l FA drug studies ( n =14; referred to as FA) or from recording sessions in which 41 traces were captured from 11 AB fish over multiple timepoints (referred to as Timepoint), were used for comparison of zERG and LabChart performance. (A) Metrics obtained from zERG are nearly identical to those obtained from LabChart. (B) Comparison of heart rate between LabChart, zERG and a manual calculation. zERG heart rate calculations are nearly identical to those obtained from a manual calculation. bpm, beats/min.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Comparison

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Correlation of zebrafish ECG traits and covariates. Zebrafish ECG traits (heart rate and intervals) are correlated with each other and with body size parameters, sex and age. The correlation matrix is derived from measurements obtained from a total of 70 AB and wild-type fish from the kcnh6a s290 and kcnh6a tb218 lines. Positive correlations are colored in blue and negative correlations in red. The color intensity and size of the circles are proportional to the correlation (i.e. a correlation of 0.80 will have a larger and darker red circle than a correlation of 0.10).

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: Derivative Assay

Journal: Disease Models & Mechanisms

Article Title: Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

doi: 10.1242/dmm.048827

Figure Lengend Snippet: Delayed ventricular repolarization in kcnh6a s290 /+ mutants. kcnh6a s290 /+ (heterozygous) mutants displayed a longer QT interval than their kcnh6a +/+ (wild-type) clutchmates ( P =0.021). Measurements were obtained from a total of 54 fish. The P -values were determined from a linear mixed model, in which the date of the ECG recording session was added as a random effect and age, ECG recording location, sex and weight were added as fixed effects; QT interval was additionally adjusted for heart rate. The QT interval plot shows the measurement uncorrected for heart rate.

Article Snippet: The commercial PowerLab system integrates a data acquisition (PowerLab) hardware device, which digitizes the ECG signals with an ECG signal amplifier (Animal BioAmp), processing the acquired data through the associated LabChart software.

Techniques: